To investigate the potential anti estrogenic effects of the relative test agents alone and in combination with curcumin, we examined i the effect on cell proliferation using the E screen assay, ii the ability to induce the Insulin Growth Factor Binding Protein 3 IGFBP 3, a known anti estrogenic marker in MCF 7 cells 32, iii the effect on alkaline phosphatase activity ALP and osteocalcin levels, two well known markers for osteoblastic differentiation and mineralization 33, iv the effect on ALP activity in HEC 1A cells, a known marker of estrogen responsiveness in endometrial cancer cells 34, 35 buy cialis canada pharmacy